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Ishikawa Fruit Tree Gardening Association (1987) Ishikawa Fruit Tree. Hashizume H, Tanase K, Shiratake K, Mori H, Yamaki S (2003) Purification and characterization of two soluble acid invertase isozymes from Japanese pear fruit. Glomb MA, Lang G (2001) Isolation and characterization of glyoxal-arginine modifications. įukuoka N, Hirabayashi H, Hamada T (2020) Oxidative stress via the maillard reaction is associated with the occurrence of internal browning in roots of sweetpotato ( Ipomoea batatas). įukuoka N, Miyata M, Hamada T, Takeshita E (2018) Histochemical observations and gene expression changes related to internal browning in tuberous roots of sweet potato ( Ipomea batatas). įukuoka N, Hamada T (2021) Effects of heat stress on the biological maillard reaction, oxidative stress, and occurrence of internal browning in Japanese radish ( Raphanus sativus L.). ĭiMarco T, Giulivi C (2007) Current analytical methods for the detection of dityrosine, a biomarker of oxidative stress, in biological samples. īianco RL, Rieger M (2002) Partitioning of sorbitol and sucrose catabolism within peach fruit. A role for phagocyte-derived oxidants in the formation of advanced glycation end products during inflammation. (95)00375-4Īnderson MM, Heinecke JW (2003) Production of N-(Carboxymethyl)lysine is impaired in mice deficient in NADPH oxidase. Bioorg Med Chem Lett 5:2161–2162Īmes JM (1992) The maillard reaction. Īl-Abed Y, Bucala R (1995) Nε-carboxymethyllysine formation by direct addition of glyoxal to lysine during the maillard reaction. Īkuta T, Zaki MH, Yoshitake J, Okamoto T, Akaike T (2006) Nitrative stress through formation of 8-nitroguanosine: insights into microbial pathogenesis. These results suggest that an increase in the Maillard reaction, attributed to an increased concentration of reducing sugars, during the maturation period may be fundamentally involved in the occurrence of this disorder through oxidative stress.Īhmed MU, Brinkmann FE, Degenhardt TP, Thorpe SR, Baynes JW (1997) N-epsilon-(carboxyethyl)lysine, a product of the chemical modification of proteins by methylglyoxal, increases with AGE in human lens proteins. Cellular examination of the phloem side of immature and mature fruits indicated that mature fruit had a stronger signal intensity than immature fruit in both reactions. Furthermore, examination of cells near the vascular bundle revealed stronger signal intensity on the phloem side compared to the xylem side. The strongest Maillard and oxidative stress reactions were observed in cells adjacent to the vascular bundle, and these reactions diminished with distance from the vascular bundle. Levels of oxidative stress and Maillard reaction products in the flesh of mature fruit were determined. Higher levels of the neutral invertase gene (PpNIV) were detected in mature fruit compared with immature fruit.

Conversely, sucrose content was inversely correlated to glucose content.

Glucose content of the flesh was higher in mature fruit with WSBF than in immature fruit without WSBF. The occurrence of WSBF disorder was rare in immature fruit, but was pronounced in mature fruit. In this study, samples of immature and mature pear fruits were assessed for the incidence of water-soaked brown flesh (WSBF), sugar content, expression level of sugar metabolism-related genes, and activity of the Maillard and oxidative stress reactions. The occurrence of water-soaked brown flesh in pear fruit is closely related to an activated biological Maillard reaction during the latter half of maturation.
